v region meaning in English
v区:参见variable region
Examples
- Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸虫单克隆杭独特型抗体np30的单链狐( scf )的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv )的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区( vh 、 vl )基因先后重组入原核表达质粒ptha90相应的位点上,中间通过一连接肽( gly在er ) 。 - Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y . after confirmed by sequncing . pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
方法:本文采用rt ? ? pcr的方法扩增jurkatt淋巴瘤细胞特异性重排的tcr可变区基因片段,克隆到真表达载体pcdna _ 3中,经序列测定无误后,碱裂解法大量提取质粒,制备dna疫苗。